In October 2009, Murat "Arbalet" Zhumashevich declared the creation of an esports organization during the Intel Extreme Masters tournament being held in Dubai. He was to provide the finances required to run the organization and also provide a place for players to train in, while starix, a Counter-Strike professional player, took up the duty of creating a lineup for the organization.

On 17 December 2009, Natus Vincere, at the time with the name of Arbalet UA, was formed, based around their Counter-Strike team, which consisted of players Ivan "Edward" Sukharev, Yegor "markeloff" Markelov, Sergey "starix" Ischuk, Arsenij "ceh9" Trunozhenko and Danylo "Zeus" Teslenko, with ZeroGravity taking the position of manager.

The abbreviation Na'Vi was inspired by the film Avatar, with its full form being decided by the fans through a competition.